Quantitation of pentobarbital in serum by enzyme immunoassay.
J Anal Toxicol
; 8(2): 59-60, 1984.
Article
em En
| MEDLINE
| ID: mdl-6371379
ABSTRACT
Pentobarbital was quantitated in serum by a homogeneous enzyme immunoassay (EMIT), and the method evaluated for accuracy and precision. A comparison was performed against the authors' modified Goldbaum ultraviolet spectrophotometric procedure. The analytical sensitivity was 2 micrograms/mL with a linearity to 10 micrograms/mL and a coefficient of variation of 6.24%. Linear regression analysis revealed a correlation coefficient of 0.977, a slope of 1.05, and an intercept of -0.07. The t test demonstrated no difference between the two methods at a level of P less than 0.01. The enzyme immunoassay also had the advantages of speed and small sample requirement.
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Base de dados:
MEDLINE
Assunto principal:
Pentobarbital
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article