Membrane 126-kilodalton phosphoglycoprotein associated with human carcinomas identified by a hybridoma antibody to mammary carcinoma cells.
Proc Natl Acad Sci U S A
; 80(5): 1332-6, 1983 Mar.
Article
em En
| MEDLINE
| ID: mdl-6572392
ABSTRACT
A large number of hybridomas producing antibodies to the membrane fraction of a human mammary carcinoma cell line have been produced. Among those hybridoma antibodies that bound to exposed surface membrane epitopes on the human mammary carcinoma cell line BT-20, one has been found to bind to all tested breast carcinoma cell lines as well as to a number of other human carcinomas by solid-phase radioimmunoassay. This hybridoma antibody, designated 10-3D2, does not bind to normal mammary epithelium, a variety of control cell lines, or selected normal human tissue homogenates. The 10-3D2 hybridoma antibody identifies by immunoprecipitation a membrane 126-kilodalton protein which is indistinguishable in different tumor cells. It is glycosylated by reference to incorporation of [3H]glucosamine and also is phosphorylated by reference to incorporation of 32P. It is present in a finely granular pattern on the cell surface of mammary carcinoma cells with concentration at the sites of apparent cell-cell contact. This 126-kilodalton phosphoglycoprotein, which we have consistently been unable to identify by immunoprecipitation of intrinsically labeled normal cells, may represent a heretofore unrecognized tumor-associated marker for human mammary carcinomas as well as certain other types of human neoplasms.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
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Neoplasias da Mama
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Glicoproteínas
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Anticorpos Monoclonais
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Neoplasias
Idioma:
En
Ano de publicação:
1983
Tipo de documento:
Article