Sequential imaging of indium-111-labeled monoclonal antibody in human mammary tumors hosted in nude mice.
J Nucl Med
; 25(5): 592-603, 1984 May.
Article
em En
| MEDLINE
| ID: mdl-6726438
ABSTRACT
Using a bifunctional chelating agent, indium-111 was attached to a monoclonal antibody 10- 3D2 , specific for a 126-kilodalton phosphoglycoprotein antigen associated with human mammary carcinoma, and was then used to localize and visualize human mammary tumors hosted in nude mice. Simultaneous tumor concentration of In-111-10- 3D2 was eight times greater than that of control I-125-MOPC-21. Uptake of F(ab')2 and Fab of 10- 3D2 was also compared. the scintigrams demonstrated that intact antibody provided the best images. Control In-111-labeled MOPC-21 and plasma did not show specific localization in the tumor. Uptake of In-111-labeled 10- 3D2 was also compared in two lines of human mammary tumors, BT-20 and HS- 578T . Imaging with 10- 3D2 was better for BT-20 than for HS- 578T . These studies demonstrated that (a) In-111-10- 3D2 can be utilized to image human mammary tumors hosted in nude mice; (b) intact antibody provided the best tumor images, although F(ab')2 had optimal target-to-background ratios for earlier imaging; and (c) different mammary tumor lines with possibly different concentrations of tumor-associated antigen showed different rates of uptake and apparent saturation with 10- 3D2 .
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Base de dados:
MEDLINE
Assunto principal:
Radioisótopos
/
Neoplasias da Mama
/
Índio
/
Neoplasias Mamárias Experimentais
/
Anticorpos Monoclonais
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article