Presence of serotonin1A (5-HT1A) receptor mRNA without binding of [3H]-8-OH-DPAT in peripheral blood mononuclear cells.
Life Sci
; 57(24): 2197-203, 1995.
Article
em En
| MEDLINE
| ID: mdl-7475972
ABSTRACT
The authors investigated the presence of serotonin receptor type 1A (5-HT1A) as labeled by the specific ligand 3H-8 hydroxy-2-(di-N-propylamino)tetralin (3H-8-OH-DPAT) in saturation experiments, and the expression of the mRNA encoding them, in human peripheral blood mononuclear cells (PBMC). In situ hybridization experiments were performed as well. The results, showing that the binding of [3H]-8-OH-DPAT to lymphocyte membranes increased linearly up to 100 nM without reaching saturation, may indicate that the 3H-8-OH-DPAT was not specifically labeling the 5-HT1A receptor. By contrast, the expression studies revealed 5-HT1A mRNA in PBMC. These findings suggest that, despite the presence of mRNA, 5-HT1A receptors are not expressed in PBMC, at least in healthy controls.
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Base de dados:
MEDLINE
Assunto principal:
Leucócitos Mononucleares
/
Receptores de Serotonina
/
8-Hidroxi-2-(di-n-propilamino)tetralina
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article