SARs do not impair position-dependent expression of a kit/lacZ transgene.
Biochem Biophys Res Commun
; 211(3): 735-41, 1995 Jun 26.
Article
em En
| MEDLINE
| ID: mdl-7541198
ABSTRACT
We have cloned and sequenced 4.2 kb of the murine c-kit promoter region. Analysis of transgenic embryos for a kit/lacZ fusion gene showed (i) an unexpected expression in the neural retina, and (ii) an unusual sensitivity to position effects. This indicates that major c-kit tissue specific regulating elements lie outside the proximal promoter. We took advantage to the intrinsic properties of the kit/lacZ transgene to investigated the potential of scaffold attachment regions (SARs) to confer position-independence on gene regulation in an in vivo context. No SAR-dependent isolation of the kit/lacZ transgene was found in the transgenic embryos, indicating a limited capacity of SAR sequences to buffer the effect of flanking sequences on transgene expression.
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Base de dados:
MEDLINE
Assunto principal:
Sequências Reguladoras de Ácido Nucleico
/
Embriologia
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Regulação da Expressão Gênica
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Receptores de Fator Estimulador de Colônias
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Proteínas Proto-Oncogênicas
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Interferon beta
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Receptores Proteína Tirosina Quinases
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article