Determination of d-amphetamine in biological samples using high-performance liquid chromatography after precolumn derivatization with o-phthaldialdehyde and 3-mercaptopropionic acid.
J Chromatogr B Biomed Appl
; 666(2): 241-50, 1995 Apr 21.
Article
em En
| MEDLINE
| ID: mdl-7633600
ABSTRACT
An HPLC method is described for the determination of amphetamine using fluorometric detection after derivatization with o-phthaldialdehyde and 3-mercaptopropionic acid. This procedure is more sensitive (detection limit 370 fmol in microdialysate buffer standards, 1.5 pmol in extracted plasma and tissue samples) than most of the previous methods described for the determination of amphetamine with HPLC-fluorescence detection. Due to the stability of the derivative it is also suitable for autosampling after manual derivatization. Investigators currently using o-phthaldialdehyde derivatization and fluorometric detection for amino acid determination should be able to rapidly implement this method.
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Base de dados:
MEDLINE
Assunto principal:
O-Ftalaldeído
/
Cromatografia Líquida de Alta Pressão
/
Dextroanfetamina
/
Ácido 3-Mercaptopropiônico
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article