Photocleavable biotin derivatives: a versatile approach for the isolation of biomolecules.
Proc Natl Acad Sci U S A
; 92(16): 7590-4, 1995 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-7638235
ABSTRACT
While the strong biotin-avidin interaction has been widely used for the detection of biomolecules, its irreversibility complicates their isolation. We report the synthesis of a photocleavable biotin derivative (PCB) which eliminates many limitations of existing methods. This reagent contains a biotin moiety linked through a spacer arm to a photocleavable moiety, which reacts selectively with primary amino groups on any substrate. In experiments using [leucine]-enkephalin as a model substrate, we show that PCB retains its high affinity toward avidin/streptavidin and allows rapid (< 5 min) and efficient (> 99%) photorelease of the substrate in a completely unaltered form. Photocleavable biotins should be useful in numerous applications involving the isolation of proteins, nucleic acids, lipids, and cells.
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Base de dados:
MEDLINE
Assunto principal:
Biotina
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article