Determination of ergosterol in organic dust by gas chromatography-mass spectrometry.
J Chromatogr B Biomed Appl
; 666(1): 77-84, 1995 Apr 07.
Article
em En
| MEDLINE
| ID: mdl-7655624
ABSTRACT
A gas chromatographic-mass spectrometric method was developed for the determination of ergosterol in organic dust. Samples were hydrolyzed under alkaline conditions, and the hydrolysate was extracted, purified on a silica-gel column, and subjected to derivatization. The limit of detection of the trimethylsilyl ether derivative of ergosterol was approximately 10 pg and that of the tert.-butyldimethylsilyl ether derivative was approximately 20 pg (injected amounts). House dust contained 6-45 micrograms ergosterol/g and air from a pig barn contained 0.2-0.3 ng ergosterol/liter. The proposed method can be used as a complement or alternative to microscopy and culturing for measuring fungal biomass in air-borne organic dust.
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Base de dados:
MEDLINE
Assunto principal:
Poeira
/
Ergosterol
/
Cromatografia Gasosa-Espectrometria de Massas
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article