Translational control of mammalian serine hydroxymethyltransferase expression.
Biochem Biophys Res Commun
; 214(2): 496-502, 1995 Sep 14.
Article
em En
| MEDLINE
| ID: mdl-7677757
ABSTRACT
We have investigated the role of an open reading frame upstream of and overlapping that coding for rabbit cytosolic serine hydroxymethyltransferase in the post-transcriptional regulation of enzyme expression. Elimination of the AUG codon initiating translation of the upstream open reading frame by site-directed mutagenesis (to an AUC codon) was assessed by a transfection assay in COS-1 cells. The mutation relieves translational repression with a 100-fold increase in expressed serine hydroxymethyltransferase activity compared to control-transfected cells and in the absence of any increase in the level of serine hydroxymethyltransferase mRNA. The upstream open reading frame may play a regulatory role in changing levels of expression of serine hydroxymethyltransferase through a translational control mechanism.
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Base de dados:
MEDLINE
Assunto principal:
Glicina Hidroximetiltransferase
/
Biossíntese de Proteínas
/
Regulação Enzimológica da Expressão Gênica
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article