IL-9 induces expression of granzymes and high-affinity IgE receptor in murine T helper clones.
J Immunol
; 154(10): 5061-70, 1995 May 15.
Article
em En
| MEDLINE
| ID: mdl-7730612
ABSTRACT
Interleukin 9 (IL-9) is a TH2 cytokine that has been shown to promote the antigen-independent growth of some mouse T helper clones. To characterize the specificity of IL-9-mediated T cell activation, we used a murine T cell clone that could grow with either IL-9 or IL-2. After differential hybridization of a cDNA library, we isolated three genes that were expressed preferentially in the presence of IL-9. Two of them correspond respectively to granzyme A and granzyme B, two proteases expressed by activated T cells. By Northern blot hybridization and functional assays, we found that IL-9 induced the expression of granzyme B in several T cell clones as well as in mast cell lines. In addition, other proteases such as the mouse mast cell proteases were also found to be expressed by IL-9-activated T cell clones. The third IL-9-induced cDNA corresponds to the alpha-chain of the high-affinity receptor for IgE. Several T cell clones expressed this IgE receptor mRNA and were able to bind IgE with high affinity. Taken together, our results indicate that IL-9 induces a mast cell-like phenotype in T cell clones.
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Base de dados:
MEDLINE
Assunto principal:
Serina Endopeptidases
/
Interleucina-9
/
Receptores de IgE
/
Linfócitos T Auxiliares-Indutores
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article