Wounding induces a series of closely related trypsin/chymotrypsin inhibitory peptides in leaves of tobacco.
Phytochemistry
; 37(4): 921-6, 1994 Nov.
Article
em En
| MEDLINE
| ID: mdl-7765662
ABSTRACT
Wounding of tobacco (Nicotiana tabacum) leaves induced the expression of acid-stable trypsin/chymotrypsin inhibitory activity. Analysis by gel filtration determined that the inhibitory activity was contained within a fraction with a native M(r) of ca 5-7 x 10(3). Using ion-exchange column chromatography, this was resolved further into two major fractions, each of which inhibited both trypsin and chymotrypsin. Reverse-phase HPLC identified a total of six peptides from both fractions and each was purified to homogeneity. Four of these peptides inhibited both trypsin and chymotrypsin, a fifth inhibited trypsin only, while the sixth inhibited chymotrypsin almost exclusively. Sequencing of the N-terminal revealed that each peptide had an identical amino acid sequence and that these proteins are similar to a series of trypsin/chymotrypsin inhibitory peptides that are expressed predominantly in the stigmas of Nicotiana alata flowers.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Plantas Tóxicas
/
Nicotiana
/
Quimotripsina
/
Inibidores da Tripsina
Idioma:
En
Ano de publicação:
1994
Tipo de documento:
Article