Typing of the granulocyte-specific NA antigens by restriction fragment length polymorphism analysis.
Br J Haematol
; 87(2): 428-30, 1994 Jun.
Article
em En
| MEDLINE
| ID: mdl-7947295
ABSTRACT
An RNA-based method has been developed to genotype donors for the granulocyte-specific alloantigens NA1 and NA2. mRNA was isolated from granulocytes, reversely transcribed into cDNA and amplified using an Fc-gamma-receptor III-1 sequence-specific primer in the polymerase chain reaction (PCR). PCR products were analysed by restriction fragment length polymorphism (RFLP) using the restriction endonuclease Taq I, which provided a distinct restriction fragment pattern corresponding to the NA alleles. 17 donors were typed by PCR-RFLP and the results were in close accordance with those obtained by serological phenotyping by granulocyte immunofluorescence and the antigen capture assay MAIGA.
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Base de dados:
MEDLINE
Assunto principal:
Polimorfismo de Fragmento de Restrição
/
Tipagem e Reações Cruzadas Sanguíneas
/
Isoantígenos
/
Antígenos de Superfície
Idioma:
En
Ano de publicação:
1994
Tipo de documento:
Article