Comparison of two techniques for measurement of in vitro killing kinetics of five antibiotics against Pseudomonas aeruginosa.
Eur J Clin Microbiol Infect Dis
; 13(5): 412-7, 1994 May.
Article
em En
| MEDLINE
| ID: mdl-8070456
ABSTRACT
The in vitro activity of ticarcillin, imipenem, gentamicin, amikacin and ciprofloxacin against six Pseudomonas aeruginosa isolates was evaluated by two time-killing curve methods:
the conventional broth technique, and another method previously described which employs a transfer filter membrane. The patterns of the killing curves obtained over a 5 h period with the two techniques were similar. In contrast to the results obtained for beta-lactam agents, the reduction of inoculum was great and increased with the concentration for aminoglycosides and ciprofloxacin. After 5 h, regrowth of the six strains tested was observed in broth with all antibiotics, whereas a bactericidal effect was observed over 24 h with the filter membrane method. Further studies are warranted to determine the reasons for this difference.
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Base de dados:
MEDLINE
Assunto principal:
Pseudomonas aeruginosa
/
Técnicas Bacteriológicas
/
Antibacterianos
Idioma:
En
Ano de publicação:
1994
Tipo de documento:
Article