Genes coding for the selenocysteine-inserting tRNA species from Desulfomicrobium baculatum and Clostridium thermoaceticum: structural and evolutionary implications.
J Bacteriol
; 176(5): 1268-74, 1994 Mar.
Article
em En
| MEDLINE
| ID: mdl-8113164
ABSTRACT
The genes (selC) coding for the selenocysteine-inserting tRNA species (tRNA(Sec)) from Clostridium thermoaceticum and Desulfomicrobium baculatum were cloned and sequenced. Although they differ in numerous positions from the sequence of the Escherichia coli selC gene, they were able to complement the selC lesion of an E. coli mutant and to promote selenoprotein formation in the heterologous host. The tRNA(Sec) species from both organisms possess all of the unique primary, secondary, and tertiary structural features exhibited by E. coli tRNA(Sec) (C. Baron, E. Westhof, A. Böck, and R. Giegé, J. Mol. Biol. 231274-292, 1993). The structural and functional properties of the tRNA(Sec) species from prokaryotes analyzed thus far support the notion that tRNA(Sec) may be an evolutionarily conserved structure whose function in the primordial genetic code was to decode UGA with selenocysteine.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA de Transferência Aminoácido-Específico
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Clostridium
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Evolução Biológica
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Genes Bacterianos
Idioma:
En
Ano de publicação:
1994
Tipo de documento:
Article