Comparison of virus neutralisation and enzyme-linked immunosorbent assay for the identification of antibodies against vesicular stomatitis (Indiana 3) virus.
Rev Sci Tech
; 12(3): 849-55, 1993 Sep.
Article
em En
| MEDLINE
| ID: mdl-8219334
ABSTRACT
Bovine, equine and swine sera from areas free from vesicular stomatitis virus (VSV) Indiana 3 (IND3)--namely Argentina, Chile, Italy and Uruguay--and endemic areas (in Brazil) were examined for anti-VSV IND3 virus antibodies in order to compare results obtained using the virus neutralisation (VN) test and liquid-phase blocking enzyme-linked immunosorbent assay (ELISA). Statistical analysis of the data showed close agreement between the two techniques (K = 0.92). The ELISA gave a higher specificity, positive predictive value and efficiency than the VN test. The ELISA offers the additional advantage of using a non-infectious antigen and can therefore be used for antibody quantification in diagnostic laboratories in VSV-free areas to support epidemiological surveillance programmes.
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Base de dados:
MEDLINE
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Testes de Neutralização
/
Vírus da Estomatite Vesicular Indiana
/
Anticorpos Antivirais
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article