Stabilization of egg phosphatidylcholine liposomes by the insertion of sulfatide.
Biochim Biophys Acta
; 1153(1): 127-31, 1993 Nov 21.
Article
em En
| MEDLINE
| ID: mdl-8241244
ABSTRACT
The characteristic effects of sulfatide and its derivatives on the stability of small unilamellar vesicles of egg phosphatidylcholine liposomes in phosphate-buffered saline were investigated by measuring the leakage of carboxyfluorescein that had been entrapped in these vesicles. We found that both the sulfate group and a long acyl chain, such as lignoceric acid, of the sulfatide are essential for the stabilization. The sulfatide derivatives that contain a somewhat shorter acyl chain such as stearic acid had no effect to suppress the leakage of carboxyfluorescein. The galactose residue of sulfatide is not essential to suppress the leakage. 1H-NMR study using a paramagnetic shift reagent demonstrated that the distribution of phosphatidylcholine in the vesicles containing sulfatide is homogeneous, which seems to contribute to the stability of the membrane.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Fosfatidilcolinas
/
Sulfoglicoesfingolipídeos
/
Lipossomos
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article