Efficient gene transfer with less cytotoxicity by means of cationic multilamellar liposomes.
Biochem Biophys Res Commun
; 196(3): 1042-8, 1993 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-8250862
ABSTRACT
A simple procedure for the preparation of cationic multilamellar vesicles (MLV) consisting of N-(alpha-trimethylammonioacetyl)-didodecyl-D-glutamate chloride, dilauroyl phosphatidylcholine, and dioleoyl phosphatidylethanolamine in a molar ratio of 122 was devised. When bacteriophage lambda DNA was encapsulated into these liposomes, entrapment efficiency was found to be nearly 100%, and digestibility of the DNA was less than 10%. Upon encapsulation of the plasmid pCH110 into cationic MLV, efficient expression was comparable to that obtained with cationic vesicles prepared by reverse-phase evaporation method (REV). Cytotoxicity of the present liposomes was less than that of cationic REV and far less than that of Lipofectin.
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Base de dados:
MEDLINE
Assunto principal:
Pentosiltransferases
/
Plasmídeos
/
DNA Viral
/
Sobrevivência Celular
/
Bacteriófago lambda
/
Técnicas de Transferência de Genes
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article