Promoter of the POU-M1/SGF-3 gene involved in the expression of Bombyx silk genes.
J Biol Chem
; 269(4): 2733-42, 1994 Jan 28.
Article
em En
| MEDLINE
| ID: mdl-8300605
ABSTRACT
To characterize the transcription regulation of the POU-M1/SGF-3 gene, we have cloned a genomic DNA fragment encompassing the whole coding region and its flanking sequences. This gene does not contain any intron. The 5'-flanking region of the gene contains several interesting motifs, such as homeodomain-binding motifs, sequences resembling the transcriptional factor Sp1-binding site, and TGTTT motifs, but lacks some of the typical transcriptional regulatory sequences, such as TATA and CCAAT boxes. Transcriptional analysis of a series of deletion mutants of the gene in the nuclear extracts prepared from the middle silk gland of 2-day-old fifth instar larvae revealed the presence of multiple cis-regulatory elements located both upstream and downstream of the initiation site. One of these elements, the homeodomain-binding element, was identified to mediate negative regulation. By mobility shift assay using the POU-M1 specific antibodies, we found that this negative element interacts with the POU-M1/SGF-3. Transcription analysis in vitro using templates mutagenized in the PB region and one of the POU-M1 antibodies indicated that the PB region is an autoregulatory element responsible for SGF-3-dependent transcriptional repression.
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Base de dados:
MEDLINE
Assunto principal:
Bombyx
/
Fatores de Transcrição
/
Proteínas
/
Regulação da Expressão Gênica
/
Regiões Promotoras Genéticas
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Genes de Insetos
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Proteínas de Insetos
/
Proteínas de Ligação a DNA
Idioma:
En
Ano de publicação:
1994
Tipo de documento:
Article