Avidin-biotin based electrochemical immunoassay for thyrotropin.
Ann Clin Biochem
; 30 ( Pt 6): 570-7, 1993 Nov.
Article
em En
| MEDLINE
| ID: mdl-8304726
ABSTRACT
A two site electrochemical enzyme immunoassay for thyrotropin (TSH) has been developed. This assay is based on the use of an immobilized capture antibody and a biotinylated second antibody. Detection is achieved via avidin labelled with alkaline phosphatase. The substrate 1-naphthyl phosphate was used and the product 1-naphthol was detected at disposable screen-printed carbon 8-electrode combs using specially designed instrumentation.
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Base de dados:
MEDLINE
Assunto principal:
Tireotropina
/
Técnicas Imunoenzimáticas
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article