Microplate technique for screening and assessing cytotoxicity of xenobiotics with Tetrahymena pyriformis.
Ecotoxicol Environ Saf
; 32(2): 159-65, 1995 Nov.
Article
em En
| MEDLINE
| ID: mdl-8575361
ABSTRACT
The microplate technique (MT) is developed and compared to the usual flask technique (FT) of Tetrahymena pyriformis GL cultures for assessing toxic effects of organic and inorganic substances on this ciliated protozoa model. Applied to the IC50 determination of 29 xenobiotics, the MT demonstrated good correlation with the FT (Y = 0.991X + 0.012, r = 0.94, P < 0.0001). The MT is rapid, easy-to-handle, inexpensive, and statistically reliable and, so, may be helpful for screening investigations of water-soluble xenobiotics.
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Base de dados:
MEDLINE
Assunto principal:
Tetrahymena pyriformis
/
Bioensaio
/
Xenobióticos
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article