Epidermal growth factor induces the differential release of GP2 and amylase from AR4-2J cells.
Cell Signal
; 7(6): 559-69, 1995 Aug.
Article
em En
| MEDLINE
| ID: mdl-8588972
ABSTRACT
Epidermal growth factor (EGF) stimulates secretion of glycoprotein 2 (GP2) in a time-and concentration-dependent manner from the AR4-2J pancreatoma cell line. Cell differentiation induced by dexamethasone treatment for 3 d, however, did not significantly alter either basal or EGF-stimulated GP2 release. Basal and EGF-stimulated GP2 release were similarly unaffected by caerulein, which promotes amylase secretion by a regulated route. A brief exposure to cycloheximide profoundly blocked EGF-evoked GP2 secretion. Furthermore, EGF-stimulated GP2 release was not accompanied by significant alterations in intracellular ionic calcium levels, in contrast to the stimulatory actions of caerulein. We conclude that EGF-stimulated release of GP2 occurs via a novel secretory pathway that is neither regulated nor constitutive as currently defined.
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Base de dados:
MEDLINE
Assunto principal:
Pâncreas
/
Glicoproteínas de Membrana
/
Fator de Crescimento Epidérmico
/
Amilases
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article