Cloned and expressed rat Ca2+-sensing receptor.
J Biol Chem
; 271(11): 5972-5, 1996 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-8626377
ABSTRACT
We have stably expressed cDNA for the rat brain Ca2+ sensing receptor in Chinese hamster ovary cells. Stimulation of phosphatidylinositol hydrolysis and arachidonic acid (AA) release displayed markedly cooperative responses to Ca2+ with Hill coefficients of 4-5. Both phosphatidylinositol and AA responses were not detected below a threshold of 1.5 mM Ca2+. Mg2+ behaved as a partial agonist with only half the maximal inositol phosphate and AA responses displayed by Ca2+ and with a more shallow concentration-response slope. The potency of Mg2+ in augmenting inositol phosphate and AA responses, in the presence of 1.5 mM Ca2+, implies that serum Mg2+ concentrations attained in clinical conditions will influence the Ca2+-sensing receptor.
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Base de dados:
MEDLINE
Assunto principal:
Cálcio
/
Receptores de Superfície Celular
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article