Genetic evidence that the RAG1 protein directly participates in V(D)J recombination through substrate recognition.
Proc Natl Acad Sci U S A
; 93(6): 2333-8, 1996 Mar 19.
Article
em En
| MEDLINE
| ID: mdl-8637873
ABSTRACT
RAG1 protein is essential for the activation of V(D)J recombination in developing lymphocytes (V, variable; D, diversity; J, joining). However, it has not been determined whether its role involves substrate recognition and catalysis. A single amino acid substitution mutation in the RAG1 gene has now been identified that renders its activity sensitive to the sequence of the coding region abutting the heptamer site in the recombination signal sequence. These results strongly imply that RAG1 interacts directly with DNA.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Recombinação Genética
/
Rearranjo Gênico do Linfócito B
/
Proteínas
/
Proteínas de Homeodomínio
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article