Two-peak kinetic curve of the chemiluminescence in phorbol-induced macrophage.

ABSTRACT

Phorbol-1,2-myristate-1,3-acetate can induce rat macrophage to emit chemiluminescence. The kinetic curve of the chemiluminescence clearly showed two peaks when a proper ratio of phorbol-1,2-myristate-1,3-acetate and macrophage were mixed. It was proved that the first one mainly came from the oxygen free radicals and the second one from NO generated in the phorbol-1,2-myristate-1,3-acetate stimulated cells by using the substitute and inhibitor of NO synthase.