A recombinant PCR approach requiring only three non-chimeric primers to generate a minigene of interest.
Genet Anal
; 12(3-4): 133-5, 1996 Jan.
Article
em En
| MEDLINE
| ID: mdl-8673737
ABSTRACT
The recombinant PCR allows construction of chimeric molecules. Here we describe this approach utilizing non-chimeric primers. Unlike previous recombinant PCR methods, this approach eliminates the need of multiple sets of primers and multiple rounds of PCR making it an economical and expeditious alternative. We have used this approach to generate an FGF-1 minigene.
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Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
/
Primers do DNA
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article