v-src interferes with the in vitro erythropoietic stimulatory ability of spleen stromal cells through repression of vascular cell adhesion molecule-1 and stem cell factor.
Exp Hematol
; 24(8): 883-7, 1996 Jul.
Article
em En
| MEDLINE
| ID: mdl-8690046
ABSTRACT
A mouse spleen stromal cell line, MSS62, can create an in vitro erythropoietic microenvironment in which development of erythropoietin-responsive progentor cells is stimulated by cell-cell contact via stem cell factor (SCF)/c-Kit and vascular cell adhesion molecule-1 (VCAM-1)/very late activation antigen-4 (VLA-4) interactions between stromal and erythroid cells. To find out the effect of src on the erythropoietic microenvironment, MSS62 cells were transfected with v-src oncogene, and its effect on erythropoietic stimulatory activity was measured. Transfectants with high v-Src activity showed reduction in erythropoietic stimulatory activity. A decrease in cell-surface VCAM-1 and SCF mRNA was accompanied by high v-Src activity. These results suggest that v-Src interferes with the erythropoietic stimulatory activity of the stromal cells through repression of VCAM-1 and SCF.
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Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Hematopoéticas
/
Genes src
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Proteína Oncogênica pp60(v-src)
/
Molécula 1 de Adesão de Célula Vascular
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Fator de Células-Tronco
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Eritropoese
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article