Molecular cloning and sequencing of cDNAs encoding insecticidal peptides from the primitive hunting spider, Plectreurys tristis (Simon).
Insect Biochem Mol Biol
; 26(5): 411-7, 1996 May.
Article
em En
| MEDLINE
| ID: mdl-8763160
ABSTRACT
Plectreurys tristis cephalothorax mRNA was isolated and amplified by PCR using degenerate primers corresponding to reverse translated mature Plt-VI toxin. An oligonucleotide corresponding to a portion of the amplified product was then used to screen a P. tristis cDNA library. The cDNAs from 10 positive clones were sequenced. Eight of these cDNAs corresponded to Plt-VI toxin, one to Plt-XI toxin, and one was very similar to Plt-VIII toxin, with the exception of a single amino acid substitution. Analysis of these cDNAs indicated that these toxins are initially synthesized as prepro-forms which undergo signal cleavage followed by additional processing at both their N- and C-termini to produce the mature products.
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Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Aranhas
/
Inseticidas
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article