Atomic force microscopy proposes a novel model for stem-loop structure that binds a heat shock protein in the Staphylococcus aureus HSP70 operon.
Biochem Biophys Res Commun
; 226(3): 730-4, 1996 Sep 24.
Article
em En
| MEDLINE
| ID: mdl-8831682
ABSTRACT
The Staphylococcus aureus HSP70 operon produces a polycistronic RNA in response to heat shock, and ORF37 is the first protein to be translated. The promoter of this operon contains a palindromic nucleotide sequence that may form a stem-loop structure. Structural analysis of the promoter regions by atomic force microscopy (AFM) revealed a quadruplet that consists of a pair of stem-loops. A novel "SL2S' (Stem-Loop-Loop-Stem) model was proposed for this structure. AFM also revealed the binding of ORF37 to the quadruplet, establishing a molecular mechanism for this heat shock gene expression; ORF37 acts as a regulator by binding to the SL2S structure in the promoter.
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Base de dados:
MEDLINE
Assunto principal:
Óperon
/
Staphylococcus aureus
/
DNA Bacteriano
/
Proteínas de Choque Térmico HSP70
/
Conformação de Ácido Nucleico
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article