The amiloride-sensitive Na+/H+ exchange antiporter and control of intracellular pH in hippocampal brain slices.
Brain Res
; 731(1-2): 108-13, 1996 Aug 26.
Article
em En
| MEDLINE
| ID: mdl-8883860
ABSTRACT
The intracellular pH, 7.54 +/- 0.03 (mean +/- S.D., n = 15), determined with the Neutral red method, of the hippocampal brain slice preparation under baseline incubation conditions is considerably more alkaline than the bath buffer pH. Neutralization by amiloride suggests that the alkalinity was due to Na+/H+ exchange antiporter activation. To characterize the brain Na+/H+ exchange antiporter we compared the inhibitory effects of MIA, amiloride and other 5-N substituted analogues on proton extrusion after acid loading by transient exposure to ammonium chloride in the isolated hippocampal brain slice preparation. The potencies of amiloride compounds on the initial recovery rate of intracellular pH after acid-loading were DMA > MIA > HMA = MHA > or = IPA-HCI > IPA > MNPA = Amil > Benzamil. The greater potency of the 5-N substituted analogs of amiloride over amiloride and benzamil strongly suggest that Na+/H+ exchange antiporter is the mechanism responsible for alkalinization in the isolated hippocampal brain slice in vitro.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Trocadores de Sódio-Hidrogênio
/
Amilorida
/
Hipocampo
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article