Separation and functional analysis of eukaryotic DNA topoisomerases by chromatography and electrophoresis.
J Chromatogr B Biomed Appl
; 684(1-2): 307-21, 1996 Sep 20.
Article
em En
| MEDLINE
| ID: mdl-8906479
ABSTRACT
DNA topoisomerases are enzymes that control DNA topology by cleaving and rejoining DNA strands and passing other DNA strands through the transient gaps. Consequently, these enzymes play a crucial role in the regulation of the physiological function of the genome. Beyond their normal functions, topoisomerases are important cellular targets in the treatment of human cancers. In this review we summarize current protocols for extracting and purifying DNA topoisomerases, and for separating subtypes and isoforms of these enzymes. Furthermore, we discuss methods for measuring the catalytic activity of topoisomerases and for monitoring the molecular effects of topoisomerase-directed antitumor drugs in cell-free assays.
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Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida
/
DNA Topoisomerases Tipo I
/
Eletroforese
/
Células Eucarióticas
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article