Purification and characterization of porcine prorelaxin.
J Biochem Biophys Methods
; 31(1-2): 69-80, 1996 Jan 11.
Article
em En
| MEDLINE
| ID: mdl-8926340
ABSTRACT
Relaxin is a two-chain 6-kDa peptide hormone. It is a member of the insulin family of peptides and is produced mainly during pregnancy to prepare the reproductive tract for birth. In the pig, relaxin is produced mainly by ovarian luteal cells. It is processed via the regulated pathway from a larger (18 kDa) precursor, prorelaxin. Protocols have been described for the purification of mature relaxin from the ovaries of pregnant gilts. Multiple forms of relaxin have been detected during isolation due to exopeptidase trimming of the peptide chains. To date, such trimming events have prevented purification of the larger relaxin precursor. Described here is a method for the isolation of milligram amounts of homogeneous and bioactive prorelaxin from porcine ovaries.
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Base de dados:
MEDLINE
Assunto principal:
Ovário
/
Precursores de Proteínas
/
Relaxina
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article