Expression and purification of the crystalline surface layer protein of Rickettsia typhi.
Microbiol Immunol
; 40(3): 233-6, 1996.
Article
em En
| MEDLINE
| ID: mdl-8934678
ABSTRACT
The crystalline surface layer (S-layer) protein (SLP) of Rickettsia typhi is known as the protective antigen against murine typhus. We previously reported a cloning and sequence analysis of the SLP gene of R. typhi (slpT) and showed that the open reading frame of this gene encodes both the SLP and a 32-kDa protein. To express only the SLP from this gene, the putative signal sequence and the 32-kDa protein portion were removed from the slpT. This protein was expressed in Escherichia coli as a fusion protein, consisting of the SLP and maltose binding protein. The recombinant protein reacted strongly with polyclonal antiserum of a patient with murine typhus.
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Base de dados:
MEDLINE
Assunto principal:
Rickettsia typhi
/
Proteínas da Membrana Bacteriana Externa
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Proteínas de Bactérias
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Proteínas de Transporte de Monossacarídeos
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Glicoproteínas de Membrana
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Regulação Bacteriana da Expressão Gênica
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Transportadores de Cassetes de Ligação de ATP
/
Proteínas de Escherichia coli
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article