Structural characterization of 15-hydroxytrichodiene, a sesquiterpenoid produced by transformed tobacco cell suspension cultures expressing a trichodiene synthase gene from Fusarium sporotrichioides.
Phytochemistry
; 43(6): 1235-7, 1996 Dec.
Article
em En
| MEDLINE
| ID: mdl-8987907
ABSTRACT
Tobacco (Nicotiana tabaccum) cell suspension cultures transformed with a gene encoding trichodiene synthase, a sesquiterpene synthase from the fungus Fusarium sporotrichioides, produced a novel sesquiterpenoid derived from the in vivo production of trichodiene. Mass and nuclear magnetic resonance spectroscopic analyses identified the new compound as 15-hydroxytrichodiene. The in vivo hydroxylation of trichodiene by transformant tobacco cell suspension cultures demonstrates that the introduction of a foreign sesquiterpene synthase gene can result in the production of novel sesquiterpenoid metabolites.
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Base de dados:
MEDLINE
Assunto principal:
Plantas Tóxicas
/
Sesquiterpenos
/
Nicotiana
/
Carbono-Carbono Liases
/
Fusarium
/
Liases
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article