vWf inhibitor detection by competitive ELISA.
J Immunol Methods
; 200(1-2): 113-9, 1997 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-9005950
ABSTRACT
An inhibitor to von Willebrand factor (vWf) was detected in the plasma from two patients with histories of mild bleeding and one patient with a severe deficiency in the Factor VIII complex using a competitive enzyme-linked immunosorbent assay (ELISA) procedure. IgG antibodies from the patients' plasmas were shown to bind to vWf immobilised on polystyrene beads by flow cytometry. The inhibitor also potentiated a recently described platelet function assay based on stirring vWf immobilised on polystyrene beads with platelet rich plasma (PRP). Upon addition of mAb IV.3, potentiation of vWf bead-induced platelet activation was lost indicating that the enhancement of platelet activation was Fc receptor-dependent. Since the ELISA described can be used to quantitate vWf and to detect inhibitors to vWf in plasma samples, the method should prove useful in differentiating acquired vWd from congenital vWd.
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Base de dados:
MEDLINE
Assunto principal:
Fator de von Willebrand
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article