Gamma linolenic acid regulates gap junction communication in endothelial cells and their interaction with tumour cells.
Prostaglandins Leukot Essent Fatty Acids
; 56(4): 307-16, 1997 Apr.
Article
em En
| MEDLINE
| ID: mdl-9150377
ABSTRACT
Tumour-endothelial cell adhesion forms a key role in the establishment of distant metastases. This study examined the effect of gamma linolenic acid (GLA), an anti-cancer polyunsaturated fatty acid (PUFA), on both the gap junction communication of human vascular endothelial cells and tumour cell-endothelial interactions. By using scrape loading of Lucifer yellow dye, we showed that GLA at non-toxic levels increased Lucifer yellow transfer, indicating improved gap junction communication. The fatty acid also corrected the communication that was reduced by the mitogenic and motogenic factor HGF/SF. GLA inhibited the tyrosine phosphorylation of connexin-43, a protein that formed gap junction in this cell. When human tumour cells were added to quiescent or HGF/SF-activated endothelial cells, the presence of GLA reduced adhesion of tumour cells to the endothelium. It is concluded that GLA reduces tumour-endothelium adhesion, partly by improved gap junction communications of the endothelium.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
Endotélio Vascular
/
Comunicação Celular
/
Neoplasias do Colo
/
Junções Comunicantes
/
Ácido gama-Linolênico
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article