Enhanced expression of glucose-6-phosphate dehydrogenase in human cells sustaining oxidative stress.
Biochem J
; 323 ( Pt 3): 801-6, 1997 May 01.
Article
em En
| MEDLINE
| ID: mdl-9169615
ABSTRACT
Recent reports have demonstrated that glucose-6-phosphate dehydrogenase (G6PD) activity in mammalian cells is necessary in order to ensure cell survival when damage is produced by reactive oxygen intermediates. In this paper we demonstrate that oxidative stress, caused by agents acting at different steps in the biochemical pathway controlling the intracellular redox status, determines the increase in G6PD-specific activity in human cell lines of different tissue origins. The intracellular level of G6PD-specific mRNA also increases, with kinetics compatible with the induction of new enzyme synthesis. We carried out experiments in which cells were exposed to oxidative stress in the presence of inhibitors of protein or RNA synthesis. These demonstrated that increased G6PD expression is mainly due to an increased rate of transcription, with a minor but significant contribution of regulatory mechanisms acting at post-transcriptional levels. These results provide new information on the defence systems that eukaryotic cells possess in order to prevent damage caused by potentially harmful oxygen derivatives.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Estresse Oxidativo
/
Glucosefosfato Desidrogenase
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article