Diazotizable supports of potential interest as affinity chromatography matrices.
J Mol Recognit
; 9(5-6): 701-5, 1996.
Article
em En
| MEDLINE
| ID: mdl-9174960
ABSTRACT
Immobilization of affinity ligands, proteins, enzymes and other functional groups by azo coupling is based on the high reactivity of the support-carrying diazonium groups towards both low- and high-molecular weight compounds containing certain groupings such as phenols, imidazole and some other heterocycles, thiols and amines. The precursor of diazonium group is a diazotizable amine on the matrix. Remarkable progress in its preparation was achieved by application of (4-amino-phenyl)-(2-sulphatoxyethyl) sulphone-type reagents for functionalization of the matrix. A similar type of amine precursor is prepared by the reaction of monosubstituted sulphanilamide with epoxide-containing matrix. The presence of a SO2 group in the para position to diazonium group of these supports (after diazotization) enhances reactivity in azo coupling. 'Reversed' azo coupling is the reaction of a matrix containing functional groups capable of reacting with diazonium groups of a ligand. Preparation of a suitable matrix and examples of diazotizable ligands are given.
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Base de dados:
MEDLINE
Assunto principal:
Cromatografia de Afinidade
/
Compostos de Diazônio
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article