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Refined structures of three crystal forms of toxic shock syndrome toxin-1 and of a tetramutant with reduced activity.
Prasad, G S; Radhakrishnan, R; Mitchell, D T; Earhart, C A; Dinges, M M; Cook, W J; Schlievert, P M; Ohlendorf, D H.
Afiliação
  • Prasad GS; Department of Biochemistry, Medical School, University of Minnesota, Minneapolis 55455, USA.
Protein Sci ; 6(6): 1220-7, 1997 Jun.
Article em En | MEDLINE | ID: mdl-9194182
ABSTRACT
The structure of toxic shock syndrome toxin-1 (TSST-1), the causative agent in toxic shock syndrome, has been determined in three crystal forms. The three structural models have been refined to R-factors of 0.154, 0.150, and 0.198 at resolutions of 2.05 A, 2.90 A, and 2.75 A, respectively. One crystal form of TSST-1 contains a zinc ion bound between two symmetry-related molecules. Although not required for biological activity, zinc dramatically potentiates the mitogenicity of TSST-1 at very low concentrations. In addition, the structure of the tetramutant TSST-1H [T69I, Y80W, E132K, I140T], which is nonmitogenic and does not amplify endotoxin shock, has been determined and refined in a fourth crystal form (R-factor = 0.173 to 1.9 A resolution).
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Toxinas Bacterianas / Superantígenos / Enterotoxinas Idioma: En Ano de publicação: 1997 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Toxinas Bacterianas / Superantígenos / Enterotoxinas Idioma: En Ano de publicação: 1997 Tipo de documento: Article