Pig lymphocytes utilise mouse MAdCAM-1 to enter fetal gut xenografts in SCID mice.
Cell Immunol
; 182(1): 38-44, 1997 Nov 25.
Article
em En
| MEDLINE
| ID: mdl-9427808
ABSTRACT
Ileocecal junction (ICJ) and proximal intestine (PI) fragments from CD45(323-) allovariant fetal pigs were grafted subcutaneously into SCID mice. The xenografts were examined 8-12 weeks later using two-color immunohistology and the ICJ, but not PI, xenografts were found to contain three types of vessels. The first (the majority) was lined with mouse endothelium (mAb 9F1+), the second was lined with pig endothelium, and the third was chimeric. The ICJ vessels were specifically lined with mouse endothelium expressing MAdCAM-1, the mucosal addressin. Vessels lined with pig endothelium alone did not express the MAdCAM-1 epitopes. Radiolabeled allovariant pig peripheral blood lymphocytes (PBL) were introduced i.v. into the xenografted SCID mice, and entry into xenografts studied. Pig PBL were occasionally seen in MECA-367+ vessel walls after 4 h and within the ICJ but not PI xenografts after 24 h. This entry was specifically blocked by coinjection of the anti-MAdCAM-1 mAb MECA-367. The results demonstrate reendothelialization of xenografts by host endothelium that expresses its own addressin and is functional for xenogenic PBL.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulinas
/
Linfócitos
/
Receptores de Retorno de Linfócitos
/
Intestinos
/
Mucoproteínas
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article