Hypoglycemia-induced seizures reduce cyclic AMP response element binding protein levels in the rat hippocampus.
Neuroscience
; 83(4): 1155-60, 1998 Apr.
Article
em En
| MEDLINE
| ID: mdl-9502254
ABSTRACT
Cyclic AMP response element binding protein (CREB) is a transcription factor that has been implicated in the activation of protein synthesis required for long-term memory. Since memory deficits are manifest following seizure, we undertook the present study to investigate the effects of hypoglycemia-induced seizure on CREB-immunoreactive neurons in several brain regions. We induced generalized seizures in male Long Evans rats (n=5) by injecting them with insulin (30 IU/kg, i.p). Animals were recovered by administration of 3 ml of 30% glucose within 5 min of the occurrence of seizure. Control animals (n=3) were injected with saline instead of insulin. All animals were perfused 90 min after recovery and the brains processed for CREB immunohistochemistry. Cell counts were determined for CREB-positive neurons using a computer-assisted program. When compared to control animals there was a 50% decrease (P<0.0001) in CREB-positive neurons in the CA1 region of the experimental animals. In the CA3 and dentate gyrus there was a 36% (P<0.001) and 25% decrease (P<0.001), respectively. Given the importance of hippocampus in memory-related processes and evidence that CREB is critical for memory formation, it is possible that seizures interfere with memory by disrupting CREB-dependent transcription.
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Base de dados:
MEDLINE
Assunto principal:
Convulsões
/
Encéfalo
/
Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico
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Hipocampo
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Hipoglicemia
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Neurônios
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article