Photodynamic tumor therapy: mitochondrial benzodiazepine receptors as a therapeutic target.
Mol Med
; 4(1): 40-5, 1998 Jan.
Article
em En
| MEDLINE
| ID: mdl-9513188
ABSTRACT
BACKGROUND:
Photodynamic therapy employs photosensitive agents such as porphyrins to treat a variety of tumors accessible to light-emitting probes. This approach capitalizes on the selective retention of porphyrins by cancer cells. Cancer cells also have elevated levels of mitochondrial benzodiazepine receptors which bind porphyrins with high affinity.METHODS:
Cultured cancer cell lines were exposed to porphyrin and porphyrin-like compounds and then irradiated with light. Cytotoxicity of this treatment was measured via clonogenic assays. Mitochondrial benzodiazepine receptor pharmacology was studied using [3H] PK11195 binding to cancer cell homogenates and isolated kidney mitochondrial membranes.RESULTS:
We show that therapeutic potencies of porphyrins correlate closely with affinities for mitochondrial benzodiazepine receptors. Sensitivities of tumor cell lines to photodynamic therapy parallel their densities of these receptors.CONCLUSION:
We propose that porphyrin photodynamic therapy is mediated by mitochondrial benzodiazepine receptors.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fotoquimioterapia
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Porfirinas
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Receptores de GABA-A
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Mitocôndrias
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Neoplasias
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article