Separation of aromatic acids, DOPA, and methyl-DOPA by capillary electrophoresis with dendrimers as buffer additives.
J Chromatogr Sci
; 36(3): 146-54, 1998 Mar.
Article
em En
| MEDLINE
| ID: mdl-9514004
ABSTRACT
Polyamidoamine starburst dendrimers with terminal carboxylate groups are used as a pseudo-stationary phase in electrokinetic chromatography for separating the selected aromatic amino acids phenylalanine, phenylglycine, homophenylalanine, and tyrosine and the catecholamines 3-(3,4-dihydroxyphenyl)alanine and 3-(3,4-dihydroxyphenyl)-2-methylalanine at different pH levels. A significant difference in analyte selectivity is observed between dendrimers of different generations and at different pH levels. At pH 7.0, the utility of the dendrimers for separating these analytes is limited by relatively low selectivity and a noisy baseline. However, good separation and selectivity are obtained with low generation dendrimers (G0.5 and G1.5) at low pH. Strong association of the solute with dendrimers is observed for high generations (G2.5, G3.5, and G5.5).
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Poliaminas
/
Materiais Biocompatíveis
/
Ácidos Carboxílicos
/
Di-Hidroxifenilalanina
/
Metildopa
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article