Substitution of Asn for Ser543 in the large fragment of Taq DNA polymerase increases the efficiency of synthesis of long DNA molecules.
FEBS Lett
; 425(2): 249-50, 1998 Mar 27.
Article
em En
| MEDLINE
| ID: mdl-9559658
ABSTRACT
Substitution of Asn for Ser543 in the large fragment of Taq DNA polymerase (Klentaq) increases several times the efficiency of synthesis of long (over 2 kbp) DNA molecules. The difference in the DNA synthesis efficiencies by the mutant and native enzymes increased with the increase in the DNA fragment length.
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Base de dados:
MEDLINE
Assunto principal:
Asparagina
/
Serina
/
DNA Viral
/
Mutagênese Sítio-Dirigida
/
Taq Polimerase
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article