FISH preimplantation diagnosis of chromosome aneuploidy in recurrent pregnancy wastage.
J Assist Reprod Genet
; 15(5): 310-3, 1998 May.
Article
em En
| MEDLINE
| ID: mdl-9604766
ABSTRACT
PURPOSE:
Our purpose was to detect aneuploidy for chromosomes 13, 16, 18, 21, 22, X, and Y in preimplantation embryos from patients with a history of unexplained recurrent miscarriage.METHODS:
Three patients with a history of unexplained recurrent spontaneous abortion were included in this study. Embryos were biopsied at the eight-cell stage, individually fixed on slides, and processed for fluorescent in situ hybridization (FISH). A multiple FISH protocol for seven chromosomes pairs (13, 16, 18, 21, 22, X, and Y) has been developed.RESULTS:
A total of 39 embryos was studied with the multiple FISH protocol developed. Successful analysis of the biopsied embryos was achieved within the time limits usually allowed in a preimplantation diagnosis program. Analysis of the blastomeres showed that 17 embryos were chromosomally normal for the probes used, 16 embryos were aneuploid, and in 6 embryos no informative results were obtained.CONCLUSIONS:
In the patients studied, a large proportion of embryos (41%) exhibited chromosomal abnormalities for the probes used. Preimplantation diagnosis to screen for chromosome abnormalities could be a feasible approach to improve the possibility of successful pregnancy in these couples.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Aborto Habitual
/
Aberrações Cromossômicas
/
Hibridização in Situ Fluorescente
/
Diagnóstico Pré-Implantação
/
Aneuploidia
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article