Sex identification of elk (Cervus elaphus canadensis), moose (Alces alces), and white-tailed deer (Odocoileus virginianus) using the polymerase chain reaction.
J Forensic Sci
; 43(3): 477-82, 1998 May.
Article
em En
| MEDLINE
| ID: mdl-9608686
ABSTRACT
We have developed a PCR-based protocol to determine the gender of tissue samples originating from elk (Cervus elaphus canadensis), moose (Alces alces) and white-tailed deer (Odocoileus virginianus). The technique simultaneously amplifies a conserved region of the sex-determining gene on the Y-chromosome (Sry) and a region of the Fragile X mental retardation gene (Fmr-1). The multiplex nature of this protocol allows the determination of gender using the Sry marker with the Fmr-1 marker providing an internal control. This technique is applicable to the enforcement of the validation tag system for game species. Data are provided from a wildlife investigation in Ontario.
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Base de dados:
MEDLINE
Assunto principal:
Análise para Determinação do Sexo
/
Fatores de Transcrição
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Cervos
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Proteínas Nucleares
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Reação em Cadeia da Polimerase
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Impressões Digitais de DNA
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Proteínas de Ligação a RNA
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Proteínas de Ligação a DNA
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Proteínas do Tecido Nervoso
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article