A rapid, small scale method for characterization of plasmid insertions in the Dictyostelium genome.
Nucleic Acids Res
; 26(13): 3317-8, 1998 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-9628938
ABSTRACT
A rapid, simple method for characterization of plasmid insertions in the Dictyostelium discoideum genome was developed. It is based on the capability of linear plasmid multimers in the insertions to recircularize efficiently in Escherichia coli cells. This recombinational recircularization of plasmid multimers provides a highly sensitive and reliable tool for determining whether individual Dictyostelium transformants resulted from restriction enzyme-mediated integration (REMI) or from recombinational integration of plasmid (RIP). The method also reveals any rearrangements in RIP insertions and provides an estimate of the vector copy number in any particular transformant.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Elementos de DNA Transponíveis
/
Genoma Fúngico
/
Genoma de Protozoário
/
Dictyostelium
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article