Precise determination of RNA-protein contact sites in the 50 S ribosomal subunit of Escherichia coli.
Biochem J
; 334 ( Pt 1): 39-42, 1998 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-9693099
ABSTRACT
RNA-protein cross-linked complexes were isolated and purified to obtain precise data about RNA-protein contact sites in the 50 S ribosomal subunit of Escherichia coli. N-terminal microsequencing and matrix-assisted laser desorption ionization MS were used to identify the cross-linking sites at the amino acid and nucleotide levels. In this manner the following contact sites of five ribosomal proteins with the 23 S rRNA were established Lys-67 of L2 to U-1963, Tyr-35 of L4 to U-615, Lys-97 of L21 to U-546, Lys-49 of L23 to U-139 or C-140 and Lys-71 and Lys-74 of L27 to U-2334.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Ribossômicas
/
Ribossomos
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RNA Ribossômico 23S
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article