Isolation of mutants deficient in acetyl-CoA synthetase and a possible regulator of acetate induction in Aspergillus niger.
Microbiology (Reading)
; 144 ( Pt 7): 1895-1900, 1998 Jul.
Article
em En
| MEDLINE
| ID: mdl-9695922
ABSTRACT
Acetate-non-utilizing mutants in Aspergillus niger were selected by resistance to 1.2% propionate in the presence of 0.1% glucose. Mutants showing normal morphology fell into two complementation groups. One class of mutant lacked acetyl-CoA synthetase but had high levels of isocitrate lyase, while the second class showed reduced levels of both acetyl-CoA synthetase and isocitrate lyase compared to the wild-type strain. By analogy with mutants selected by resistance to 1.2% propionate in Aspergillus nidulans, the properties of the mutants in A. niger suggest that the mutations are either in the structural gene for acetyl-CoA synthetase (acuA) or in a possible regulatory gene of acetate induction (acuB). A third class of mutant in a different complementation group was obtained which had abnormal morphology (yellow mycelium and few conidia); the specific lesion in these mutants has not been determined.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Acetato-CoA Ligase
/
Aspergillus niger
/
Mutação
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article