Effect of mutations in the small envelope protein of hepatitis B virus on assembly and secretion of hepatitis delta virus.
Virology
; 251(1): 176-86, 1998 Nov 10.
Article
em En
| MEDLINE
| ID: mdl-9813213
ABSTRACT
The gene coding for the small (S) envelope protein of hepatitis B virus was mutated to identify sequences important for the envelopment of the nucleocapsid during morphogenesis of hepatitis delta virus (HDV) virions. This study was focused on a domain of the S protein that is exposed in the cytoplasm during synthesis and thereby represented a good candidate for interaction with the viral nucleocapsid during virion assembly. The mutations consisted of deletion/insertions spanning the entire cytosolic domain of S between amino acid residues 24 and 80. Although the expression of mutants clustered between residues 59 and 80 could not be obtained, we demonstrated that a large part of the cytosolic loop, from residues 29-47 and 49-59, does not contain motifs essential for production of hepatitis B virus subviral particles or HDV virions. However, deletion of residues 24-28 led to the synthesis of S protein mutant, which was competent for secretion of subviral particles but deficient for production of HDV. We concluded that the sequence between Arg-24 and Ile-28 located at the carboxyl boundary of the transmembrane signal I for S contains residue or residues important for HDV particle assembly.
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Base de dados:
MEDLINE
Assunto principal:
Precursores de Proteínas
/
Vírus Delta da Hepatite
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Proteínas do Envelope Viral
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Vírus da Hepatite B
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Montagem de Vírus
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Antígenos de Superfície da Hepatite B
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Mutação
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article