[Interaction of 17beta-estradiol with histones. Effect of the hormone on partially reconstructed mucleohistones]. / Vzaimodeistvie 17beta-éstradiola s gistonami. Deistvie gormona na chastichno rekonstruirovannye nukleogistony

Binding of 17beta-estradiol with histones of calf thymus and the influence of the hormone on heart denaturing of partially reconstructed nucleohistones were investigated. By the degree of the hormone binding histone fractions could be arranged in the following order: F2a greater than F3 greater than F1 greater than or equal F2b. Addition of 17beta-estradiol gave rise to increase of the first and a corresponding decrease of the second melting phase of partially reconstructed nucleohistones F2a, F3 and F2b. Addition of the hormone to the partially reconstructed nucleohistone F1 gave rise to the opposite changes in the heat denaturing profile.